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Department of Viral Glycoproteins
Institute of Biochemistry
Short link:
https://eeris.eu/
ERIF-2000-000D-1048
747
Visits
The Viral Glycoproteins group was founded in 2002 with the aim to apply the methods developed in the Institute for the study of glycoprotein folding, to a more specific but highly challenging field of molecular biology, the viral envelope packing. Our group has been focused on studying iminosugar derivatives as antiviral agents against both HBV and HCV. Viruses that encode one or more glycoproteins are likely to depend on the host-cell mechanisms of protein folding and quality control for proper...
Norica
Branza-Nichita
norica.nichita@biochim.ro
PhD, Senior resercher
SCIENTIFIC TEAM
2
Catalin LAZAR
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Costin-Ioan POPESCU
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Cell Culture Facilities
RI Domain of activity
Drug Screening Facilities
RI Domain of activity
Research Data Management Plan:
No information available
Access Policy to Research Infrastructure and Related Services:
No information available
Research Services
Anti-viral drug screening
SERVICE DESCRIPTION:
We are able to perform drug screening on different viral systems: HBV, HCV, BVDV
SERVICE PERSONS:
Costin-Ioan POPESCU
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Catalin LAZAR
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Detection, quantitation and purification methods for HBV and HCV
SERVICE DESCRIPTION:
SERVICE PERSONS:
Costin-Ioan POPESCU
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Catalin LAZAR
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Purification of viral antigens (HBV, HCV)
SERVICE DESCRIPTION:
SERVICE PERSONS:
Costin-Ioan POPESCU
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Catalin LAZAR
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Research Equipment
Preparative Ultracentrifuge
CATALOG NAME:
Optima XPN 100
DESCRIPTION:
The Optima XPN generates centrifugal forces for the separation of particles. Classified S, it can be used with all currently manufactured Beckman Coulter rotors for floor-model preparative ultracentrifuges. In our lab we have the following Beckman rotors: SW41Ti, SW32Ti, SW60Ti, SW 28, NVT 65, TYPE 100Ti, TYPE 70Ti
PRODUCER:
Beckman Coulter
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (1855.85 kb)
Laboratory Automation Workstation
CATALOG NAME:
Biomek 3000
DESCRIPTION:
Biomek 3000 Laboratory Automation Workstation offers a liquid handling platform that's as advanced as it is flexible. By integrating all aspects of liquid handling into a single, automated system, Biomek 3000 delivers unparalleled flexibility, expandability and performance.
PRODUCER:
Beckman Coulter
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (155.47 kb)
Class II Biological Safety Cabinet
CATALOG NAME:
HERAsafe KS
DESCRIPTION:
HERAsafe KS safety cabinets incorporate a range of revolutionary ergonomic design make safety cabinet operation simpler, easier and more comfortable, creating an inherently safer working environment. • Motorized aerosol-tight front window allows precise and easy operation of the front window, seals the inner chamber for secure contamination protection for samples and personnel in stand-by mode. • Hinged front window allows full access to the interior for thorough cleaning/disinfection as well as easy load and unload of the work space • Slanted front window and arm rests support ergonomic working posture and minimize operator fatigue • Mobile control unit allows users to keep hands within the sterile work area while parameter settings are changed
PRODUCER:
Thermo Scientific
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (3587.71 kb)
CO2 Incubator
CATALOG NAME:
HERAcell 150i
DESCRIPTION:
The CO2 incubator HERAcell® 150 i is a laboratory device for preparing and cultivating cell and tissue cultures. The device allows the simulation of the special physiological ambient conditions for these cultures due to the exact control of: • temperature, • CO2 content, • O2/N2 content, • and increased relative humidity.
PRODUCER:
Thermo Scientific
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (8076.27 kb)
Refrigerated Benchtop Centrifuge
CATALOG NAME:
Microfuge 22R
DESCRIPTION:
Includes: F241.5P rotor Microfuge® 22R Refrigerated Microcentrifuge Features: - Fast and Cool - High g-Force for Fast and Optimal Pelleting Maintenance-free rotor spins up to 14,000 rpm (21,920 x g) Ideal for pelleting of DNA, RNA and Proteins; Cell Virus Isolation Maintains 4º C at maximum speed to protect precious samples Stand-by cooling Easy-to-use control knobs and easy-to-read display Comes complete with a 24-place aerosol-tight, autoclavable fixed-angle rotor Quiet operation — < 58 dB - Interchangeable Rotors for Application Flexibility All rotors come standard with aerosol-tight lids minimizing contamination from broken or leaking tubes All rotors are autoclavable at 121º C
PRODUCER:
Beckman Coulter
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (1674.9 kb)
Inverted Microscope
CATALOG NAME:
Axio Vert. A1
DESCRIPTION:
Choose from all standard contrasting techniques, including DIC, to investigate your cell cultures. Axio Vert.A1 produces brilliant images to answer your questions. Axio Vert.A1 is the only system in its class with such a large range of features, compact enough in fact to sit directly beside your incubator. Look into the very essence of your research while keeping your cell culture in its own protected environment.
PRODUCER:
Ziezz
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (5555.92 kb)
Protein Gel Electrophoresis Systems
CATALOG NAME:
XCell SureLock® Mini-Cell
DESCRIPTION:
The XCell SureLock®Mini-Cell’s unique design allows you to run mini-gels quickly, easily, and leak-free without any clamps or grease. The set-up time is only 30 seconds; just drop the Buffer Core and the Gel Tension Wedge into the Lower Buffer Chamber, insert the gels, and pull the Gel Tension Wedge forward. The perfect no-leak seal results in no mess and consistent performance. Tough polycarbonate construction of the unit boosts durability. Retractable plugs, recessed jacks, and a specially designed lid enhance user safety.
PRODUCER:
Invitrogen
PRODUCTION YEAR:
2014
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (440.72 kb)
Semi-dry protein blotting system
CATALOG NAME:
Trans-Blot® SD Semi-Dry Transfer Cell
DESCRIPTION:
The Trans-Blot® SD semi-dry transfer cell allows fast, efficient, economical blotting without a buffer tank or gel cassettes. Features and Benefits Transfers take place in as little as 15–60 min Minimal buffer requirements save money Capacity to transfer up to four Mini-PROTEAN® precast or handcast gels, or three Criterion™ gels Single-step locking system allows simple, speedy setup Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary Safety cover breaks the electrical current when lifted, preventing electrical shock
PRODUCER:
Bio-Rad
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (193.29 kb)
Wet/Tank Protein Blotting System
CATALOG NAME:
Mini Trans-Blot® Cell
DESCRIPTION:
The Mini Trans-Blot module accommodates two cassettes for electrophoretic transfer. The Mini Trans-Blot module is useful for blotting either protein or nucleic acid from both agarose and acrylamide gels. It is also capable of blotting isoelectric focusing gels from horizontal electrophoresis cells, or DNA and RNA gels from the Mini-Sub® submarine electrophoresis cell.
PRODUCER:
Bio-Rad
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Nucleic acids blotting equipment
CATALOG NAME:
Model 785 Vacuum Blotter
DESCRIPTION:
The Model 785 vacuum blotter is a nucleic acid transfer apparatus that uses low vaccuum to transfer DNA or RNA from agarose gels onto nylon membranes in as little as 90 minutes. This vacuum blotter simplifies every step of the blotting process. Its easy assembly reduces setup times for your experiments, while the vacuum regulator accurately controls vacuum pressure to give you consistent results.
PRODUCER:
Bio-Rad
PRODUCTION YEAR:
2010
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (436.5 kb)
Nucleic acids hybridization oven
CATALOG NAME:
HC-3000 HybriCycler Hybridization Oven
DESCRIPTION:
Compact size saves valuable benchtop space—measures only 20D x 10.5W x 16.25 in.H. · Carousel constructed of clear 0.38 in.-thick beta-blocking acrylic and polycarbonate; rotates at 11rpm for consistent saturation of samples · Touch-sensitive keypad and microprocessor ensure accurate temperature output from ambient to 80°C; readout displays on LED · Drawer-style tray under rocker wheel contains spill up to 300mL and pulls out for easy cleanup of spilled media · Holds up to eight 35 x 150mm, four 35 x 300mm, eight 50mL bottles, or one 70 x 300mm bottom (with carousel removed)
PRODUCER:
UVP
PRODUCTION YEAR:
2008
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (710.7 kb)
Thermomixer
CATALOG NAME:
Thermomixer comfort
DESCRIPTION:
Features: Mixing, heating, and cooling in one instrument for high flexibility All common vessels and plate formats from 5 µL to 50 mL by different Eppendorf SmartBlocks for standard applications Excellent 2DMix-Control technology results in excellent sample mixing
PRODUCER:
Eppendorf
PRODUCTION YEAR:
2005
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (2675.02 kb)
Automated Cell Counter
CATALOG NAME:
Countess ™ Automated Cell Counter
DESCRIPTION:
The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.
PRODUCER:
Invitrogen
PRODUCTION YEAR:
2014
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (1831.58 kb)
Integrated high throughput screening platform
CATALOG NAME:
DESCRIPTION:
Liquid handling automation workstation: BiomekFXp (Beckman Coulter) with 96 multichannel pipetting head, Span-8 and a pin-tool system. Microplate washer : ELx405 (Biotek) Cell incubator: Cytomat 48 Hotel (Thermo) Multimodal microplate reader: SpectraMax Paradigm (Molecular Devices): absorbance, luminescence, fluorescence, polarized fluorescence, BRET, FRET, TR-FRET Liquid handling automation workstation: Biomek 3000 (Beckman Coulter)
PRODUCER:
Beckman Coulter
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Phone:
+4(021) 2239069
Fax:
+4(021) 2239068
Splaiul Independentei
,
296
,
Bucharest
060031
,
Bucuresti - Sector 6
Romania
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[T: 0.7883, O: 357]
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