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Department of Molecular Cell Biology
Institute of Biochemistry
Short link:
https://eeris.eu/
ERIF-2000-000C-1034
925
Visits
The Department of Molecular Cell Biology pursues research on the molecular mechanisms of protein maturation within the endoplasmic reticulum, and in particular the processes accompanying the ER- associated protein degradation. The Department has a cell culture facility, a microscopy platform including confocal microscopes, a flow cytometry platform for analysis and cellular sorting.
Stefana
Petrescu
stefana.petrescu@biochim.ro
Dr.
SCIENTIFIC TEAM
7
Petruta ALEXANDRU
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Gabriela CHIRITOIU
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Simona GHENEA
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Gabriela NEGROIU
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Florentina PENA
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Ioana POPA
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Livia SIMA
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Cell Culture Facilities
RI Domain of activity
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
RI Domain of activity
Biomedical Imaging Facilities
RI Domain of activity
Protein synthesis and degradation , Cell biology of protein secretion
RI Domain of activity
Research Data Management Plan:
No information available
Access Policy to Research Infrastructure and Related Services:
No information available
Research Services
Identification of biomarkers by Fluorescence Microscopy
SERVICE DESCRIPTION:
We provide full service starting with the project design and sample preparation. For advanced users the Department offers support for co-localization studies, protein-protein interactions, techniques such as FRET and FRAP using confocal microscopy. The service includes image and data analysis for light microscopy. Live cell imaging techniques enabling to view and record image data of cellular processes are also available.
SERVICE PERSONS:
Simona GHENEA
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Ioana POPA
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Cell analysis and cell sorting by FACS
SERVICE DESCRIPTION:
Our flow cytometry facility offers assistance in experimental design, sample acquisition and data analysis. BD FACSCalibur (1-laser, 3 colors) and FACSVerse analyzers (3-laser, 8 colors) are dedicated for assays such as: immunophenotyping, DNA content, cell cycle analysis, apoptosis, cell proliferation, phagocytosis, intracellular antigen measurement, phosphoprotein expression, and cytokine detection. The FACSVerse instrument has an automatization module allowing high throughput sample acquisition. Services include fluorescence-associated cell sorting by BD FACSAria III (5 lasers, 18 colors) instrument into tubes, multiwell plates, or on slides using the Automatic Cell Deposition Unit.
SERVICE PERSONS:
Livia SIMA
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Analysis of cell processes with Tissue FAXS imaging cytometry system
SERVICE DESCRIPTION:
The Tissue FAXS iPlus imaging systems from Tissue Gnostics is available in our Department for quantitative analysis of histological samples (Histo Quest), and live or fixed cell samples (Tissue Quest). The automatic control of microscope stage by TissueFAXS Plates/Slides modules allows scanning and reconstitution of entire sample areas for both image analysis and archiving purposes. Samples can be investigated in bright field light (histology staining) using the PixelLink camera or fluorescence using the PCOPixelFly camera. Our services include cytometry statistical analysis of cell subpopulations based upon specific marker quantitation on DAPI, FITC, TxRed, and Cy5 channels.
SERVICE PERSONS:
Livia SIMA
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Gabriela NEGROIU
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Expert tissue culture and gene and protein expression service
SERVICE DESCRIPTION:
Our trained researchers provide advice and know-how in cultivating your cells from frozen vials to flasks. We provide project advice for establishing clonal cell lines from mixed populations. In terms of gene and protein expression, we offer know-how in gene cloning, site-directed mutagenesis, gene cloning, and transfection of mammalian cells. Expressed proteins are characterized by pulse-chase and immunoprecipitation, SDS-PAGE and Western blot, in our laboratories.
SERVICE PERSONS:
Simona GHENEA
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Gabriela NEGROIU
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Florentina PENA
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Gabriela CHIRITOIU
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Petruta ALEXANDRU
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Research Equipment
Confocal microscopy system
CATALOG NAME:
LSM 710
DESCRIPTION:
Confocal microscopy system for images acquisition and analysis equipped for fluorescence microcopy.
PRODUCER:
Carl Zeiss
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (3126.87 kb)
Inverted microscope with "ViVA Tome"
CATALOG NAME:
AxioObserver Z.1 microscope
DESCRIPTION:
AxioObserver Z.1 research microscope with "VivATome", dual camera system, HXP 120 and Colibri
PRODUCER:
Carl Zeiss
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (1930.29 kb)
Research microscope with Apotome
CATALOG NAME:
AxioImager M2 upright researches microscope with Apotome 2 system
DESCRIPTION:
AxioImager M2 upright researches microscope with Apotome 2 system , Dapi,FITC,Cy3,DSred,Cy5 detection
PRODUCER:
Carl Zeiss
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (37 kb)
Stereomicroscope
CATALOG NAME:
SteREO Discovery.V20
DESCRIPTION:
Stereomicroscope with fluorescence module
PRODUCER:
Carl Zeiss
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (5573.06 kb)
Flow cytometer analyser
CATALOG NAME:
FACSVerse3L
DESCRIPTION:
The BD FACSVerse™ flow cytometer supports cell analysis for research applications using up to 10 parameters.
PRODUCER:
BD Biosciences
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Sorter Flow Citometer
CATALOG NAME:
FACS Aria III5L
DESCRIPTION:
Sorter Flow citometer with 5 lasers able to analyse and sort up to four cell populations simultaneously.
PRODUCER:
BD Bioscience
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Benchtop ultracentrifuge
CATALOG NAME:
Optima MAX-XP
DESCRIPTION:
The Optima MAX-XP Ultracentrifuge is a power-packed solution that delivers fast, efficient separations from samples as small as 175 μl up to 32.4 ml,and at speeds up to 150,000 RPM and more than 1,000,000 x g.
PRODUCER:
Beckman Coulter
PRODUCTION YEAR:
2011
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
High throughput Cell Analysis System
CATALOG NAME:
Tissue FAXS
DESCRIPTION:
The automated workstation for fluorescence. TissueFAXS is the optimal choice for scanning and analysis of samples in the fluorescence mode and this with up to 10 different channels and at by using any objective of choice. The combination of high quality microscopes (Zeiss, Leica, Nikon), fast computer hardware and high-resolution screens with TissueFAXS scanning software and TissueQuest analysis software brings automation into your lab. TissueFAXS is the equivalent to flow cytometry in tissue.
PRODUCER:
TissueGnostics
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Protein Gels Electrophoresis Systems
CATALOG NAME:
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell
DESCRIPTION:
The versatile, easy-to-use Mini-PROTEAN Tetra Cell is a vertical mini gel electrophoresis system designed for fast, simple setup, facilitating rapid sample optimization, method development, and targeted protein analysis.
PRODUCER:
BioRad
PRODUCTION YEAR:
2010
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Protein wet transfer system
CATALOG NAME:
The Mini Trans-Blot® cell
DESCRIPTION:
The Mini Trans-Blot® cell provides rapid, high-quality blotting of Mini-PROTEAN® handcast and precast gels. A component of the Mini-PROTEAN Tetra electrophoresis system, the Mini Trans-Blot cell accommodates two gel holder cassettes for electrophoretic transfer of mini format gels run in the Mini-PROTEAN Tetra cell.
PRODUCER:
BioRad
PRODUCTION YEAR:
2010
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Class II Biological Safety Cabinet
CATALOG NAME:
Heraeus HS12
DESCRIPTION:
Especially designed for work with dangerous biological substances Optimum contamination protection High product and personal/ operator protection from contamination Vertical laminar air flow and air intake (air curtain) across front opening Modular design for flexible, individually orientated use Safety tested in accordance with BS 5726
PRODUCER:
Heraeus
PRODUCTION YEAR:
2002
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Class II Biological Safety Cabinet
CATALOG NAME:
Heraeus LaminAir HB2448
DESCRIPTION:
Especially designed for work with dangerous biological substances Optimum contamination protection High product and personal/ operator protection from contamination Vertical laminar air flow and air intake (air curtain) across front opening Modular design for flexible, individually orientated use
PRODUCER:
Heraeus Instruments
PRODUCTION YEAR:
2000
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (6670.4 kb)
CO2 incubator
CATALOG NAME:
REVCO ELITE II
DESCRIPTION:
REVCO ELITE II is designed to create a stable, reliable environment for cell culture applications.Solid stainless steel interior improves resistance to contamination and features clean-weld corners. Triple-wall construction incorporates fiberglass insulation and enhances temperature uniformity and control. Walls and plenum components are formed from high-quality corrosion-resistant stainless steel. Reinforced construction permits stacking. The exterior features a scratch-resistant paint finish.
PRODUCER:
Revco
PRODUCTION YEAR:
2008
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (983.38 kb)
Phone:
+4(021) 2239069
Fax:
+4(021) 2239068
Splaiul Independentei
,
296
,
Bucharest
060031
,
Bucuresti - Sector 6
Romania
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