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Department of Ligand-Receptor Interactions
Institute of Biochemistry
Short link:
https://eeris.eu/
ERIF-2000-000U-1038
491
Visits
The Department of Ligand-Receptor Interactions pursues research on the molecular mechanisms of proteins involved in pathological processes, in particular the mode of action of Lactoferrin as immunomodulatory and anti-infectious molecule. Characterization of nanostructure-based biological active compound interaction with target cells are also important aspects currently addressed by our group.
Anca
Roseanu
roseanua@gmail.com
PhD, Senior researcher
SCIENTIFIC TEAM
5
Paula FLORIAN
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Madalina ICRIVERZI
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Magda MOISEI
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Anca ROSEANU
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Mihaela TRIF
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Cell Culture Facilities
RI Domain of activity
Micro and Nanotechnology Facilities
RI Domain of activity
Drug screening facilities
RI Domain of activity
Research Data Management Plan:
No information available
Access Policy to Research Infrastructure and Related Services:
No information available
Research Services
Anti-infections drug screening
SERVICE DESCRIPTION:
We are able to performe drug screening on different experimental systems of infections: viral (HBV, BVDV) and inflammation (stimulated monocytes/macrophages)
SERVICE PERSONS:
Paula FLORIAN
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Magda MOISEI
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Madalina ICRIVERZI
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Cytotoxicity and biocompatibilities tests
SERVICE DESCRIPTION:
In vitro studies of different drug delivery systms (nanoparticles, liposomes, thin films) effect on target cells (cell viability, proliferation, adhesion, morphology) using colorimetric, flow cytometry and immunofluorescence microscopy assays.
SERVICE PERSONS:
Paula FLORIAN
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Mihaela TRIF
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Magda MOISEI
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Madalina ICRIVERZI
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Lipid nanostructure formulation and drug encapsulation
SERVICE DESCRIPTION:
Obtaining and in vitro characterization of different lipid nanostructure formulations : size, shape, controlled release, interaction with target cells (binding, internalization, localization)
SERVICE PERSONS:
Mihaela TRIF
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Magda MOISEI
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In vitro evaluation of macrophage activation and of pro-inflammatory molecules secretion
SERVICE DESCRIPTION:
Detection of IL-6, TNF-alfa and other pro and anti-inflammatory cytokines by ELISA
SERVICE PERSONS:
Anca ROSEANU
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In vitro evaluation of nanoparticles designed for cancer treatment using murine melanoma cell line
SERVICE DESCRIPTION:
Cytotoxicity evaluation using colorimetric method and flow cytometry. Adhesion and cell morphology analysis based on fluorescence microscopy. Nanoparticles-cell interaction by flow cytometry and WB.
SERVICE PERSONS:
Anca ROSEANU
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In vitro assays for testing the antitumoral potential of nanoparticles
SERVICE DESCRIPTION:
Apoptosis induced by nanoparticles-flow cytometry; effect on cell cycle-flow cytometry; intracellular traffic and cellular signaling involved in antitumoral effect-flow cytometry, fluorescence microscopy, WB
SERVICE PERSONS:
Anca ROSEANU
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Research Equipment
Laminar flow cabinet
CATALOG NAME:
ASALAIR 1200 FA
DESCRIPTION:
Asalair Carbo fume cupboard model 1200 F.A. is a suction hood with molecular filtration, equipped with activated carbon filter. It assures an excellent protection both of the operator and the environment, it holds in its activated carbon filter all harmful molecules to respiration and envinroment. It’s not suitable like protection for the products manipulated into it from the external contamination.
PRODUCER:
ASAL SRL Italy
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (931.4 kb)
Zeta Potential Analyzer
CATALOG NAME:
Zeta Potential Analyzer
DESCRIPTION:
Measures electrophoretic mobility particles in media of low dielectric constant, high viscozity or high salt molarity zeta potential
PRODUCER:
Brookhaven Instrument Corporation
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (2344.64 kb)
Autotitrator
CATALOG NAME:
BI-ZTU Autotitrator
DESCRIPTION:
Determines automatically zeta potential as a function of pH, added surfactant or salt; measurement of isoelectric point.
PRODUCER:
Brookhaven Instrument Corporation
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (598.69 kb)
Liposomat
CATALOG NAME:
Liposomat
DESCRIPTION:
Dual pumping system for the removal of detergent from solutions of mixet lipid/detergent micelles.
PRODUCER:
Harvard Apparatus
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (213.47 kb)
Rotavapor
CATALOG NAME:
RV 10 control
DESCRIPTION:
PRODUCER:
IKA
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (7537.98 kb)
Refrigerated Benchtop Centrifuge
CATALOG NAME:
Microfuge 22R
DESCRIPTION:
F241.5P rotor;Fast and Cool - High g-Force for Fast and Optimal Pelleting Maintenance-free rotor spins up to 14,000 rpm (21,920 x g) Ideal for pelleting of DNA, RNA and Proteins; Cell Virus Isolation Maintains 4º C at maximum speed to protect precious samples
PRODUCER:
Beckman Coulter
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (1674.9 kb)
Wet/Tank Protein Blotting System
CATALOG NAME:
Mini Trans-Blot® Cell
DESCRIPTION:
The Mini Trans-Blot module accommodates two cassettes for electrophoretic transfer. The Mini Trans-Blot module is useful for blotting either protein or nucleic acid from both agarose and acrylamide gels. It is also capable of blotting isoelectric focusing gels from horizontal electrophoresis cells, or DNA and RNA gels from the Mini-Sub® submarine electrophoresis cell.
PRODUCER:
Bio-Rad
PRODUCTION YEAR:
2013
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (680.31 kb)
Thermomixer
CATALOG NAME:
Thermomixer comfort
DESCRIPTION:
Mixing, heating, and cooling in one instrument for high flexibility All common vessels and plate formats from 5 µL to 50 mL by different Eppendorf SmartBlocks for standard applications Excellent 2DMix-Control technology results in excellent sample mixing
PRODUCER:
Eppendorf
PRODUCTION YEAR:
2005
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (2675.02 kb)
Mini Extruder
CATALOG NAME:
Avanti Mini Extruder
DESCRIPTION:
Prepare large, unilamellar vesicles (LUVs) by extrusion in an easy, rapid, & efficient manner. Generate uniform populations of unilamellar liposomes without the use of solvents or detergents.
PRODUCER:
Avanti Polar Lipids
PRODUCTION YEAR:
2008
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (928.79 kb)
Phone:
+4(021) 2239069
Fax:
+4(021) 2239068
Splaiul Independentei
,
296
,
Bucharest
060031
,
Bucuresti - Sector 6
Romania
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[T: 0.852, O: 370]
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