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DEPARTMENT OF ENZYMOLOGY
Institute of Biochemistry
Short link:
https://eeris.eu/
ERIF-2000-000J-1037
559
Visits
The Department of Enzymology is dedicated to the study of the structure-function relationship of signaling enzymes, with emphasis on protein tyrosine phosphatases. Signaling enzymes are analyzed from different directions: substrate specificity, enzyme kinetics, specific inhibitors, physiological relevance, regulatory interactions, signaling characteristics, etc. The group has experience in the production, isolation, purification, and characterization of recombinant proteins expressed in prokar...
STEFAN EUGEN
SZEDLACSEK
stefan@biochim.ro
Dr.
SCIENTIFIC TEAM
5
Rodica Aura BADEA
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Aura Elena IONESCU
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Adina Gabriela PUIU
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Stefan SZEDLACSEK
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Andrei Mihai VASILESCU
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Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
RI Domain of activity
Cell Culture Facilities
RI Domain of activity
Biochemistry, chromatography, enzymology, physical chemistry.
RI Domain of activity
Type Of RI:
Distributed RI
RI Life Cycle Status:
Active RI
Research Data Management Plan:
No information available
Access Policy to Research Infrastructure and Related Services:
No information available
Research Services
Bacterial and mammalian protein expression and purification
SERVICE DESCRIPTION:
In the recent years the necessity to obtain recombinant protein production has increased greatly. Proteins have multiple uses in different fields from biotechnology to medicine and research. If you are interested to investigate the role of a protein, such as: structure, function, protein interactions etc., the Department of Enzymology has all equipments necessary in order to obtain your desired protein. We have the facilities to express the proteins both in prokaryotic and eukaryotic system and to obtain them with a high degree of purity.
SERVICE PERSONS:
Stefan SZEDLACSEK
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Aura Elena IONESCU
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Rodica Aura BADEA
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Adina Gabriela PUIU
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Andrei Mihai VASILESCU
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Proteome and Post-Translational Modification (Tyrosine Phosphorylation) Analysis
SERVICE DESCRIPTION:
We provide service and assistance regarding sample preparation and data interpretation of protein mixtures.
SERVICE PERSONS:
Aura Elena IONESCU
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Adina Gabriela PUIU
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Andrei Mihai VASILESCU
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Enzyme kinetic analysis
SERVICE DESCRIPTION:
We can perform kinetic analysis for a given enzyme. Kinetic parameters (specific activity, turnover rate, Michaelis constant) and inhibition constants for a set of inhibitors can be determined.
SERVICE PERSONS:
Stefan SZEDLACSEK
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Research Equipment
Nanodrop 2000
CATALOG NAME:
Nanodrop 2000 Spectrophotometer
DESCRIPTION:
The patented sample retention system of the NanoDrop 2000 and 2000c spectrophotometers allows a sample to be pipetted directly onto an optical measurement surface. Once the measurement is complete, the surfaces are simply wiped with a lint-free lab wipe. The NanoDrop 2000c integrates the sample retention system with cuvette capability. NanoDrop 2000 Micro-volume sample size (0.5-2.0μL) Easy to use—pipet sample directly onto the pedestal No dilutions required, even for highly concentrated samples Fast measurement time of less than 5 seconds Full spectral output (190 - 840 nm) Preconfigured methods for A260, A280, labeled DNA or Proteins, and much more User-friendly software that includes custom methods and data export capabilities
PRODUCER:
Thermo Scientific
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
10.001 € – 50.000 €
DATA SHEET:
Nanodrop 3300
CATALOG NAME:
Nanodrop 3300 Spectrophotometer
DESCRIPTION:
Thermo Scientific NanoDrop 3300 fluorospectrometers are used by life science laboratories to verify selective concentrations of DNA, RNA, and protein. Utilizing a patented sample retention system it minimizes the mass detection limit while increasing sensitivity, allowing measurement of samples as low as 1 picogram/µl of dsDNA. The flexibility of the NanoDrop 3300 allows users to work with multiple brands of assays, giving them the freedom to select the best assay for a specific workflow. NanoDrop 3300 Fluorospectrometer features: Measures a wide variety of fluorescence assays: RiboGreen, PicoGreen, GFP, BFP mutants, Hoechst, 4-MU, Quantum Dots, OPA, DyLite, AlexaFluor and many more Sample size as small as 1μL UV, blue and white LED excitation, covering a broad wavelength range No filter changes and no expensive monochromator Ability to run diverse and customizable applications Ability to measure multiple fluorophores from a single sample Spectral and quantitative data
PRODUCER:
Thermo Scientific
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
AKTA BASIC
CATALOG NAME:
AKTABASIC UPC10 FRAC 920
DESCRIPTION:
PRODUCER:
AMERSHAM BIOSCIENCES
PRODUCTION YEAR:
2006
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
AKTA PRIME
CATALOG NAME:
AKTA PRIME PLUS
DESCRIPTION:
ÄKTAprime plus is a lab-scale chromatography system that performs simple purifications of tagged and untagged proteins as well as antibodies. Optimal results with HiTrap, HiPrep and HiLoad prepacked columns. Specific and quick methods for tagged protein purification. Simple-to-use with preprogrammed templates covering all common techniques.
PRODUCER:
G.E. HEALTHCARE
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Typhoon
CATALOG NAME:
Typhoon FLA 9500
DESCRIPTION:
Typhoon FLA 9500 is a variable mode laser scanner with modular access to the optical components, providing both versatile and flexible imaging for precise quantitation of proteins, nucleic acids, and other biomolecules. Versatility: imaging of multifluorescent-, chemifluorescent-, radioisotope-labeled, and colorimetric samples High resolution and quantitation: a pixel resolution of up to 10 µm and a linear signal response over five orders of magnitude provides precise quantitation in gels, blots, tissue sections, and arrays High sample throughput: scanning area of 40 × 46 cm enables simultaneous imaging of up to 20 gels or blots, measuring 10 × 8 cm in size. This facilitates comparisons among blots, and reduces workload and waiting time Flexibility: optimized performance for new applications by adapting the system with stages, detectors, filters and lasers 2-D DIGE imaging: simultaneously image two 2-D DIGE gels for differential expression studies Visible and infrared fluorescence imaging: optional near infrared excitation for imaging IRDye™ and other infrared dyes The system provides several imaging modes at any time, including fluorescence and chemifluorescence, filmless autoradiography, and digitization of colorimetrically-stained samples (e.g., Coomassie™ blue and silver stain). It is also adequate for quantitative chemiluminescent imaging that does not require maximum sensitivity.
PRODUCER:
GE Healthcare
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Multitron Pro
CATALOG NAME:
Multitron Pro
DESCRIPTION:
A professional incubation shaker for any application. Applications: Protein expression in bacteria Molecular biology (e.g. mini- and maxipreps)
PRODUCER:
INFORS HT
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Olympus Microscope
CATALOG NAME:
OLYMPUS CX31
DESCRIPTION:
The CX31 fixed specification binocular biological microscope is ideal for teaching and routine applications. All main controls are located close together, enabling operation with minimal movements. Ease of use is further enhanced by the integrated Abbe condenser, which increases the visibility and contrast of the specimen under different magnifications without time-consuming adjustments. Olympus UIS2 objectives ensure bright, clear observation images with excellent flatness. Olympus digital camera UCMAD3 is used for efficient digital imaging and documentation on protein crystal formation.
PRODUCER:
Olympus
PRODUCTION YEAR:
2004
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Nikon Microscope
CATALOG NAME:
SMZ1500
DESCRIPTION:
The SMZ1500 is one of Nikon's most advanced stereomicroscopes, designed with life scientists in mind. It covers a zoom range from 0.75x to 11.25x, and offers sophisticated accessories that rival that of advanced compound microscopes. This allows the scientist to see any specimen, from macro views to high-magnification micro visualization.
PRODUCER:
Nikon
PRODUCTION YEAR:
2009
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
French Press
CATALOG NAME:
FRENCH PRESS
DESCRIPTION:
PRODUCER:
Thermo Electron Corporation
PRODUCTION YEAR:
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Eppendorf Centrifuge
CATALOG NAME:
5417R
DESCRIPTION:
PRODUCER:
Eppendorf
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
HETTICH CENTRIFUGE
CATALOG NAME:
Micro 220
DESCRIPTION:
PRODUCER:
Hettich
PRODUCTION YEAR:
2008
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
BECKMAN CENTRIFUGE
CATALOG NAME:
Avanti J25I
DESCRIPTION:
PRODUCER:
Beckman Coulter
PRODUCTION YEAR:
2006
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
HETTICH CENTRIFUGE 2
CATALOG NAME:
Universal 320R
DESCRIPTION:
PRODUCER:
Hettich
PRODUCTION YEAR:
2008
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
MULTITRON 2
CATALOG NAME:
MULTITRON 2
DESCRIPTION:
PRODUCER:
INFORS HT
PRODUCTION YEAR:
2007
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
FLUOstar Omega Microplate reader
CATALOG NAME:
FLUOstar Omega Microplate reader
DESCRIPTION:
Details The FLUOstar Omega is a versatile multi-mode microplate reader for five detection modes: fluorescence intensity (including FRET), luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen®/AlphaLISA®. Features Full-spectrum UV/Vis absorbance measurements in less than 1 second or filter-based absorbance Measurement in up to 384-well microplate format Top and bottom reading Plate shaking and uniform incubation up to 45 °C Well-scanning and real-time kinetic monitoring High-precision injectors with simultaneous reagent injection and detection Includes multi-user control and MARS data analysis software
PRODUCER:
BMG Labtech
PRODUCTION YEAR:
2012
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
DATA SHEET:
Download data sheet file (723.14 kb)
PCR- Thermo Cycler
CATALOG NAME:
DNA Engine Dyad- Peltier Thermal Cycler
DESCRIPTION:
DNA Engine Dyad Specifications Thermal range: –5.0 to 105°C, but no more than 30°C below ambient temperature (10–100°C, but not more than 23°C below ambient temperature for the Slide Chambers unit) Accuracy: ±0.3°C of programmed target at 90°C, NIST-traceable Thermal uniformity: ±0.4°C well-to-well within 30 seconds of arrival at 90°C (for most Alpha units; see specifications for individual Alpha units) Ramping speed: Up to 3°C/sec for all single- and dual-block Alpha units; Up to 1.2°C/sec for the Slide Chambers unit Sample capacity: Varies with installed Alpha unit Line voltage: 200–240 VAC Frequency: 50–60 Hz Power: 1600 W maximum Fuses: Two 6.3 A, 250 V, 5 x 20 mm Displays: One 1/4 size VGA screen (320 x 240), 16 colors Ports: One 9-pin RS-232 serial port One ethernet port Weight: 11 kg ( base only) Size: 48 x 29 x 15 cm (l x w x h, base only
PRODUCER:
BioRad
PRODUCTION YEAR:
2010
COMMISSIONING DATE:
PRICE RANGE (at the time of purchase)
less than 5.001 €
DATA SHEET:
Phone:
+4(021) 2239069
Fax:
+4(021)2239068
https://www.biochim.ro/group-enzymology/
Splaiul Independentei
,
296
,
Bucharest
060031
,
Bucuresti - Sector 6
Romania
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[T: 1.0439, O: 408]
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