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Functional Explorations Laboratory

SERVICE DESCRIPTION: The Laboratory includes an 8-bed stationary for short-term monitoring and six functional exploration laboratories, designed and fully equipped to allow complex cardiovascular and neurological examinations: - Electroencephalography and electromyography (EEG-EMG) - Syncope and other autonomic disorders (SINC) - Cardiac and vascular imaging (ECHO) - Electrocardiography (ECG) - Stress testing (STRESS) - Metabolic disease testing (METAB-DZ).

SERVICE PERSONS:

Serban ALINA

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Immunology Laboratory

SERVICE DESCRIPTION: The activity of the Laboratory is based on xMAP technology for simultaneous detection of more than 500 markers in suspension or on solid support, accompanied by ELISA, CLIA, and nephelometric techniques. These equipments allow simultaneous determination of qualitative and quantitative aspects of a large number of biomarkers, intracellular signals, cytokines, chemokines, and growth factors, metabolic markers, nuclear receptors and transcription factors. The Laboratory includes the following devices: - xMAP 3D technology and Microarray (on microchips) - automatic analyzers based on techniques such as: ELISA, CLIA, ECLIA, nephelometry, turbidimetry, spectrophotometry, coagulometry and aggregometry - complete line of electrophoresis / blotting, automatic electrophoretic system for protein separation and identification.

SERVICE PERSONS:

Minodora DOBREANU

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Adina HUTANU

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Liliana DEMIAN

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Cytology Laboratory

SERVICE DESCRIPTION: The Laboratory includes devices for cell separation / genetic modification / culture / analysis: 4-laser flow cytometer, cell separation systems, ultracentrifuge, electroporation system, inverted microscopes, cell counters, basic facilities.

SERVICE PERSONS:

Minodora DOBREANU

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Doina MANU

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Chromatography and mass spectrometry laboratory

SERVICE DESCRIPTION: The Laboratory is equipped with LC-MS/ MS QTOF triple quadrupole time of flight. QTOF detection in the case of CL has gained great popularity in biomedical research laboratories across Europe, due to its outstanding performance, being present in Romania only in few research centers. The model available in CCAMF is the first of its kind in Romania.

SERVICE PERSONS:

Silvia IMRE

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Amalia MIKLOS

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Valentin ION

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Molecular Biology / Genetics Laboratory

SERVICE DESCRIPTION: The Next Generation Sequencing technology availablr in this Laboratory enables sequencing of the entire genome and/or exons in order to identify novel mutations involved in the development of various diseases. Patterns of genetic risk will be established through multiple approaches: - genetic linkage studies - genetic association studies - genome-wide association studies (GWAS) A new direction is also the study of non-coding RNAs. The Laboratory is equipped with the latest technology: - automatic nucleic acid separator - traditional PCR and real-time qPCR systems - capillary electrophoresis sequencing system with 24 capillaries, with diagnostic ability - next generation sequencing system - microarray gene sequencing system - analysis of amplification products by electrophoresis.

SERVICE PERSONS:

Claudia BĂNESCU

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Valeriu MOLDOVAN

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Microscopy laboratory

SERVICE DESCRIPTION: The Laboratoy allows performing histochemical analysis of tissue samples. The Laboratory includes the following devices: tissue processing equipments, state-of-the-art confocal microscope - two fluorescence microscopes, LASER microdissection system.

SERVICE PERSONS:

Simona GURZU

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EVIDENCE Investigator

CATALOG NAME: EV 3602

DESCRIPTION: Many years of development and the expertise of highly qualified scientists have gone into the creation of Biochip Array Technology (BAT) - a multi-analyte testing platform facilitating the simultaneous quantitative or qualitative detection of a wide range of analytes from a single sample. Biochip Array Technology provides a unique platform for assessment of biological samples in a rapid, accurate and easy to use format and users can have the utmost confidence in this proven technology. Evidence Investigator is the world’s first platform with the unique ability to consolidate immunoassay and nucleic acid testing. A chemically activated 9x9mm ceramic biochip acts as a solid phase reaction vessel. Biochips are pre-fabricated with discrete test regions (DTR’s); a different antibody/oligonucleotide is immobilised at each spatially distinct DTR. Up to 49 individual DTR’s can be arrayed on to a single biochip with one biochip per sample used to generate multiple results simultaneously. This eliminates the need to run costly, time consuming and sample intensive singleplex. The biochip detection is based on a chemiluminescent signal emitting light, without heat, as a result of a chemical reaction. The light emitted is simultaneously detected and quantified using a CCD camera. Applications: • Academic Research • Drug Development (CRO’s and Pharma) • Molecular Research and Diagnostics • Forensic and Clinical Toxicology

PRODUCER: RANDOX BIOSCIENCES - Molecular Diagnostics

PRODUCTION YEAR: 2015

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Luminex FLEXMAP 3D

CATALOG NAME: 40-014 FLEXMAP 3D System

DESCRIPTION: Among multiplex xMAP technology analyzers, FLEXMAP 3D is the most innovative flow cytometry based system that allows multiplexing by determining up to 500 parameters of a single sample quickly and precisely through the combination of differentially dyed fluorescent microsphere sets and an innovative instrument design. Features such as automated probe height adjustment, simplified routine maintenance operations and an intuitive software interface make FLEXMAP 3D® a flexible and user-friendly multiplexing platform capable of performing a variety of protein and nucleic acid applications. The new xPONENT® 4.0 software controls the FLEXMAP 3D® system and offers interfacing options for laboratory information systems (LIS) as well as automation. - can process a large number of information in a very short time; for a 96- and 384-well plate and 500 parameters the required time is 45 minutes, the final number of results being 144,000/ hour. - is flexible with a very broad menu of predefined panels and/ or reagents for custom kits useful for applications in immunoassay, of RNA and DNA nucleic acids, or ligand-receptors. - the 3D FLEXMAP platform enables a high working speed without compromising flexibility and performance by automating sample pipetting and maintenance, and its connectivity to computer systems - ensures accuracy and reproducibility - the system has two class I lasers and a high-speed processor for analyzing the acquired data. The platform has a broad menu which includes settings for both research and diagnosis. • profile for 34 parameter cardiac markers • profile for 149 intracellular signal parameters • profile for cytokines, chemokines, and growth factors with approximately 180 parameters • metabolic marker panel of 15 parameters • nuclear receptor and transcription factor panel with 51 parameters • gene expression profile of 26 parameters • panel for autoimmune diseases • panel for genetic testing (coagulation, cystic fibro

PRODUCER: Merk Millipore

PRODUCTION YEAR: 2015

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UHPLC-QTOF system

CATALOG NAME: Flexar 10 UHPLC system coupled with 4600 QTOF mass spectrometer

DESCRIPTION: See the attached datasheet.

PRODUCER: Perkin-Elmer and Sciex

PRODUCTION YEAR: 2015

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Download data sheet file (1982.63 kb)

Echocardiograph

CATALOG NAME: Vivid E9 BT12®

DESCRIPTION: The echocardiograph, unique in Romania at this level of concomitant facilities, is conceived for bidimensional transthoracic and transesophageal adult, pediatric, and neonatal cardiac examination. The device is also suitable for vascular and transcranial examinations, as well as for contrast and interventional echography. The echocardiograph disposes of the XD Clear technology, which offers high image resolution and allows highly accurate measurements, as well as of a set of softwares for advanced quantifications. The device is equipped with: • one matrix transducer (M5S-D), build using the pure wave technology, designed for cardiac adult, pediatric and fetal echography, transcranial examination, stress echocardiography, contrast echocardiography with left ventricular contrast, renal echography; • one linear transducer (ML6-15-D), designed for vascular, breast, soft tissues, musculoskeletal, thyroid, scrotal, and rodent echography; • one convex transducer (4C-D), designed for abdominal, obstetrics-gynecology, urology, vascular, fetal cardiac, pelvis, and renal echography. Standard examination modes include: • 2D mode, with automatic tissue optimization, which can be manually implemented by pushing a single button; automatic optimization can also be activated dynamically by selecting he CTO (continuous tissue optimization) option; • M-mode, anatomic M-mode, curved anatomic M-mode, color M-mode, and color anatomic M-mode; • pulsed wave/HPRF, continuous wave, and color Doppler; • spectral tissue and color tissue Doppler; • myocardial deformation quantification by speckle tracking (AFI – Automatic Function Imaging – option); the results can be presented in the “bullseye” manner; • contrast echography (LVO contrast option); • Q analysis; • B-flow for vascular imaging. The echocardiograph: • disposes of Z-scores for pediatric examinations; • can be updated for stress echocardiography; • can be updated for 3D echocardiog

PRODUCER: GE Healthcare

PRODUCTION YEAR: 2015

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12-lead electrocardiograph

CATALOG NAME: MAC 5500®

DESCRIPTION: This 12-lead electrocardiograph offers the possibility of using 3 additional leads. The device is highly sensitive in recognizing paced rhythms, and disposes of a number of specialized softwares – acute coronary syndrome prediction, QT-interval analysis. The device is also suitable for performing signal-averaged ECG, and disposes of a dedicated software for measuring late ventricular potentials and for signal-averaged P-wave analysis. The device can also perform vectorcardiographic analysis, disposing of a dedicated software for vectorcardiographic signals (P, QRS, ST-T vectors/loops).

PRODUCER: GE Healthcare

PRODUCTION YEAR: 2015

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12-lead Holter ECG monitoring systems

CATALOG NAME: CardioMem 3000®; CardioDay®

DESCRIPTION: The Functional Explorations Laboratory disposes of five 12-lead Holter ECG monitoring systems that allow up to 48-hours of continuous 12-lead ECG monitoring. The software is designed to: - classify and edit QRS events, and rank the events based on their severity; - detect paced rhythms; - recognize atrial fibrillation; - perform complex ST-segment analysis; - perform complex, beat-to-beat QT-interval analysis; - perform heart rate variability analysis in both time and frequency domains; - perform T wave alternans analysis; - perform heart rate turbulence analysis; - perform deceleration capacity assessment.

PRODUCER: GE Healthcare

PRODUCTION YEAR: 2015

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QuantStudio™ 12K Flex Real-Time PCR System, OpenArray™ block, Accufill™ System

CATALOG NAME: QuantStudio™ 12K Flex Real-Time PCR System with AccuFill, OpenArray™ block, laptop Catalog number: 4471086

DESCRIPTION: Applications: Genotyping, SNP QC and screening, Digital PCR for rare-allele detection, MicroRNA profiling, Biomarker confirmation and screening, Gene expression quantification, GWAS follow-up studies, SNP sample tracking for biobanking, SNP fingerprinting for ancestry, Marker-assisted selection (MAS), Quantitative trait locus (QTL) mapping, Pathogen detection

PRODUCER: Thermo Fisher Scientific

PRODUCTION YEAR: 2014

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Applied Biosystems 7500 Dx Fast Real-Time PCR System

CATALOG NAME: Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, with laptop computer Catalog number: 4406984

DESCRIPTION: Genotyping and Genomic Profiling, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, High Resolution Melting (HRM) is available on Fast Real-Time. The system yields high-quality results in 30 minutes.

PRODUCER: Thermo Fisher Scientific

PRODUCTION YEAR: 2010

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Applied Biosystems 3500xL Dx Genetic Analyzer (24-Capillary)

CATALOG NAME: 3500xL Dx Genetic Analyzer Catalog number: A27856

DESCRIPTION: It is based on Sanger sequencing and has applications in de novo sequencing and resequencing, microsatellite analysis, MLPA, LOH, MLST, and SNP confirmation or screening and fragment analysis, validating NGS discoveries May be used for the human identification application, mitochondrial sequencing, genome fingerprinting, etc.

PRODUCER: Thermo Fisher Scientific

PRODUCTION YEAR: 2015

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Ion Proton System for Next-Generation Sequencing

CATALOG NAME: Ion Proton™ System Catalog number: 4476610 coupled with Ion OneTouch™ 2 System Catalog number: 4474779

DESCRIPTION: It is a benchtop sequencing system with applications in Human scale genome sequencing, Exome sequencing, Whole transcriptome sequencing or RNA-Seq, Small genome sequencing, Gene sequencing, ChIP sequencing, De novo sequencing, Methylation analysis, Gene expression by sequencing, Small RNA and miRNA sequencing

PRODUCER: Thermo Fisher Scientific

PRODUCTION YEAR: 2015

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iPrep™ Purification Instrument

CATALOG NAME: iPrep™ Purification Instrument Catalog number: IS10000

DESCRIPTION: Automation system for RNA extraction and DNA extraction, that can process up to 12 samples in 30 minutes. It ensures sample integrity with prefilled, sealed reagent cartridges enabling the extraction of RNA and DNA from viral, blood, tissue gDNA, buccal swabs and forensic samples.

PRODUCER: Thermo Fisher Scientific

PRODUCTION YEAR: 2011

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Flow cytometer with cell sorter

CATALOG NAME: BD FACSAria III ™ Flow cytometer with cell sorter

DESCRIPTION: BD FACSAria™ cell sorter sets a new standard for high-performance flow cytometry . The flow cytometer identifies multiple cell populations in a single sample. This maximizes the information derived from limited sample amounts and increases the cost-effectiveness of tests since it can bring together various information (equal to the number of channels of detection) about hundreds of thousands of cells in a suspension. Applications for cell analysis and sorting highlights: 1. Cell separation – up to 4 pure cell lines, concomitantly 2. Immunophenotyping (surface markers and intracellular markers , measurement of circulating rare cells ) 3. 3. Cellular functions, apoptosis/ cell viability, cell cycle analysis, cell proliferation BD FACSDiva software helps you movefrom analysis to sorting. The sorter is equipped with 405nm, 488nm, 561nm, and 633nm lasers and is capable of up to 9 color simultaneous detection. Populations can be sorted in up to 4 tubes which can also be temperature controlled by use of an available waterbath heated sorting block. Populations or single cells may also be sorted directly onto slides, or into standard or customized plates.

PRODUCER: BD Biosciences

PRODUCTION YEAR: 2015

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Confocal microscope

CATALOG NAME: Confocal microscope Leica TCS SP8

DESCRIPTION: Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast, eliminating out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. Applications: confocal scanning (laser scanning images) of fluorescence-marked living and fixed specimens as well as for quantitative measurements in the area of life science. Highlights :  Confocal Laser Scanning Microscopy (CLSM) is one of a series of methods to generate slices from microscopic samples by means of optics.  True Confocal Scanning (TCS) is a technique, where only a single, diffraction limited spot is illuminated and observed at a time.  incorporates a motorised inverted DMI 6000 microscope, which has 4 objectives.  Up to four fluorophores plus a bright field setting can be imaged in parallel or sequentially. There are four interactive emission detectors, enabling the user flexibility in choosing the wavelengths to detect; two of which are highly sensitive (GaAsP) allowing the imaging of faint signals.  The sample stays intact, and the slicing may be repeated many times.  The benefit of confocal imaging is a dramatically increased contrast by removal of out-of-focus haze. Z-sequences of optical slices (3D image stacks) are sources for subsequent rendering as anaglyphes, depth-coded maps or 3D movies.  CO2 and temperature controlled incubator for live cells that can be put on the stage

PRODUCER: Leica Microsystems

PRODUCTION YEAR: 2015

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Cardiopulmonary exercise testing system

CATALOG NAME: eBike EL®; CardioSoft®; Cortex Metalyzer 3B®; Responder 2000®

DESCRIPTION: The cardiopulmonary exercise testing system includes the following devices: bad-like stationary bicycle for pedaling in semi-sitting position, with adjustable tilting, adapted for concomitant echocardiographic examination (eBike EL®, GE Healthcare); dedicated system for the recording and interpretation (CardioSoft®, GE Healthcare) of effort electrocardiogram; ergospirometry system (Cortex Metalyzer 3B®, GE Healthcare) and analysis software (MetaSoft®, GE Healthcare); automatic biphasic external defibrillator (Responder 2000®, ). The system ensures automatic blood pressure measurement. The system performs: automatic and manual QRS detection and analysis; automatic detection of cardiac arrhythmias; complex ST-segment analysis – ST-segment amplitude, slope, integral, index, ST/HR slope, ST/HR loops, ST/HR index; T wave alternans analysis during stress testing; vectorcardiographic analysis. The system also allows: post-testing E, J, and post-J points selection and analysis; cardiopulmonary analysis during stress testing; VO2max, FIO2, FEO2, METS, AT, RR, VE/VO2, and PETO2 measurement. The system includes a software dedicated to ergospirometic analysis in athletes.

PRODUCER: GE Healthcare; Cardiac Science

PRODUCTION YEAR: 2015

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Elisa Dynex DSX

CATALOG NAME: DSX 4 Incubator System

DESCRIPTION: Dynex DSX fully automated ELISA analyzer, with 4-plate processing system that is capable of performing multiple assays per plate which provides flexible configuration. Is an open system analyzer which can runs ELISA assays for qualitative and quantitative detection of different analytes, from different manufacturers, for immunology, infectious disease, hormone, allergy, blood-chemistry, drugs-of-abuse testing, food safety, forensics and environmental testing. The system is designed to run up to 96 sample in a continuous flow from the beginning to results. To assure pipetting precision, all the samples and reagents are delivered using custom-designed disposable pipette tips. Pipette precision and accuracy measures: Precision Accuracy for sample <3% above 10 μl; ± 2% above 10 μl accuracy. For reagent <3% above 50 μl; ± 2% above 50 μl Reader Specifications: photometric range: 0.000-3.000 OD, spectral range 405-690 nm Sensors for fluid level, clot detection, tip detection and ejection.

PRODUCER: DYNEX Technologies, Inc.

PRODUCTION YEAR: 2015

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BN ProSpec

CATALOG NAME: OVLI12

DESCRIPTION: The BN ProSpec is a dedicated, compact system that offers a consolidated menu of specialty and routine reagents for reliable plasma protein testing. The BN ProSpec System uses our proven nephelometric technology for the determination of a large variety of assays which support the management of disease states including: gammopathies / immune system, , kidney disease, inflammation, autoimmune/rheumatoid diseases, cardiovascular risk / acute cardiac care, chronic alcohol abuse, allergic diseases, nutritional assessment, coagulation disorders, anemia/iron metabolism, complement activity, as well as innovative markers such as monoclonal kappa and lambda free light chains, Cystatin C and Carbohydrate-Deficient Transferrin (CDT).

PRODUCER: Siemens Healthcare Diagnostics

PRODUCTION YEAR: 2015

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COBAS INTEGRA 400 plus

CATALOG NAME: 04922859001

DESCRIPTION: The Cobas Integra 400 Plus is an advanced integrated system for diagnostic clinical chemistry testing, a random and continuous selective automated analyser. Classic chemistry, electrolytes, specific proteins, therapeutic drug monitoring, drugs of abuse, and thyroid hormone testing are consolidated into one system with one reagent cassette design. More than 140 applications for all types of sample matrices are measured with one of four different measuring technologies on single platform: • absorbance photometry: enzymes and substrates • turbidimetry: specific proteins, drugs of abuse • fluorescence polarimetry: therapeutic drugs, thyroid tests • ion-selective electrode potentiometry: Na+, K+, Cl+ and Li+

PRODUCER: Roche Diagnostics GmbH

PRODUCTION YEAR: 2014

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IMMULITE 2000 XPi

CATALOG NAME: 6950476

DESCRIPTION: The IMMULITE 2000 XPi has the best-in-class reliability you expect from the IMMULITE family of products. The IMMULITE 2000 XPi system is a continuous random access immunoassay analyzer with a maximum throughput of 200 tests per hour. This design increases workflow and enhances productivity for medium- to high-volume immunoassay laboratories by incorporating specialty and allergy testing into one platform. . The extensive IMMULITE 2000 XPi menu includes assays in these disease states: adrenal/pituitary, 3gallergy, anemia, bone metabolism, cardiovascular, diabetes, growth, infectious disease, inflammation markers, metabolic function, reproductive endocrinology, thyroid function, tumor markers, autoimmune, Therapeutic Drug Monitoring (TDM), veterinary.

PRODUCER: Siemens Healthcare Diagnostics

PRODUCTION YEAR: 2015

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SYSMEX XS-800i

CATALOG NAME: 5-differential haematology analyser with flourescence technology

DESCRIPTION: Principles: Fluorescent Flow Cytometry for WBC-differential, for NRBC counting and reticulocyte measurement; Precise red blood cell count and volume detection for HCT measurement; Advanced sheath flow impedance technology for PLT, RBC evaluation; Cyanide-free SLS method for Hgb measurement. Parameters: WBC- total white blood cells count; NEUT - neutrophils, absolute and percentage count; LYMPH - lymphocytes, absolute and percentage count; MONO - monocytes, absolute and percentage count; EO - eosinophils, absolute and percentage count; BA - basophils, absolute and percentage count; RBC – red blood cells count; HGB – hemoglobin; HTC – hematocrit; MCV – mean corpuscular volume; MCH – mean corpuscular hemoglobin; MCHC – mean corpuscular hemoglobin concentration; RDW-CV- red blood cell distribution width; RDW-SD- red blood cell distribution width – standard deviation; PLT – platelets; PDW - platelet distribution width; MPV – mean platelet volume; P-LCR% - platelet large cell ratio (volume >12 fl); PCT - plateletcrit; 3 histograms (PLT, RBC, WBC); 1 scattergram (DIFF- white blood cells)

PRODUCER: Sysmex Corporation, 1-5- 1 Wakinohama-Kaingandori, Chuo-ku, Kobe 651-0073, Japan

PRODUCTION YEAR: 2015

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